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of Na+ /K+ -ATPase and bicarbonate-dependent ionic pump The pump protein is located mainly on the lateral plasma membranes [Joyce, 2003] Although, like the keratocytes, the corneal endothelium is derived from neural crest, the endothelial cell characteristics are different from those of keratocytes The human corneal endothelium comprises a monolayer of polygonal cells, which are arrested in G1 phase in vivo and do not normally replicate to replace dead or injured cells [Joyce et al, 1996a,b] This relative lack of cell division results in a physiological reduction of cell density of about 03 05% per year [Bourne and Kaufman, 1976; Hollingsworth et al, 2001] Scattered evidence, however, suggests the potential for some mitotic events in human corneal endothelium Mitotic gures were observed in vivo by specular microscopy after rejection reaction of a corneal graft [Laing et al, 1984].

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7 Adding PDF417 Symbols to Crystal Reports - PDF417 Fontware ...
The software includes a file called U25MoroviaPDF417FontEncoder4.dll , which is specially crafted to provide Crystal Reports with PDF417 encoding functions.

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Protocol 4.4. Derivation of Mouse Embryos Feeder (MEF) Cells Reagents and Materials Sterile Pregnant mice dated by detection of vaginal plug (= day 0) MEF medium (see Section 4.3.1) Freezing medium: MEF medium with 20% DMSO PBSA: D-PBS without Ca2+ and Mg2+ Trypsin/EDTA: 0.05% trypsin, 0.5 mM EDTA in Hanks BSS without Ca2+ and Mg2+ Centrifuge tubes, 15 mL Freezing vials (cryotubes) Dissection instruments Syringe and 18G and 21G needles Procedure (a) Euthanize female mice on E13.5 of pregnancy. (b) Isolate embryos and wash once in PBSA: (i) Remove uterus from mouse and dissect out individual embryos [Freshney, 2005]. (ii) Remove the head and visceral organs (liver, heart, lungs, etc). (iii) Wash the carcasses twice in PBSA. (c) Transfer the carcasses to 3-cm-diameter bacteriological dishes, with two in each dish. (d) Mince two embryo carcasses together into ne pieces, using 2 crossed scalpels and working in a very small volume of PBSA. (e) Add 1 mL of trypsin/EDTA per embryo

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hydroxylation of, 441 ozonation of, superacidic, 447 2,3-Dimethylbutane alkylation with, 242 chlorination of, 586, 587 ozonation of, superacidic, 447 2,3-Dimethyl-2-butene, epoxidation of, 453 2,3-Dimethylbutene, formation of, in dimerization of propylene, 729 3,3-Dimethylbutene, skeletal rearrangement of, 175 Dimethyl carbonate, manufacture of, 384, 386 cis-l,2-Dimethylcyclobutane, ring opening of, 661 Dimethylcyclobutene, rearrangement of, 191 Dimethylcyclohexanes epimerization of, 163 oxidation of, 444 Dimethylcyclohexenes isomerization of, 186 stereochemistry of hydrogenation of, 623-625 Dimethylhexanes, aromatization of, 53 1,3-Dimethylnaphthalene, alkylation of, 252 7,7 -Dimethylnorbornene epoxidation of, 451 hydroboration of, 318 oxymercuration of, 330 3,7-Dimethyl-l-octene, polymerization of, 761 2,4-Dimethyl-l,3-pentadiene, in cycloaddition, 334 2,4-Dimethylpentane, autoxidation of, 429 Dimethyl terephthalate, 514 Diols, in cyclialkylation, 266 1,2-Diols, formation of, in bis-hydroxylation, 467-470,497 1,3-Diols, in Prins reaction, 228 1,3-Dioxacyclohexanes, in Prins reaction, 229 1,2-Dioxetanes, 462, 464, 465 Dioxiranes epoxidation with, 453, 497, 523 hydroxylation of alkanes with, 435 oxidative cleavage with, 489 Diphenylacetylene hydroamination of, 340 hydrocyanation of, 301 hydrogenation of, 674 metallation of, 607 oxidation of, 489 oxymercuration of, 330 reduction of, 676 2,3-Diphenyl-2-butenes, stereochemistry of hydrogenation of, 623 1,I-Diphenylethylene, as initiator, in anionic polymerization, 741

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